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unmethylated gpppg  (New England Biolabs)


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    New England Biolabs unmethylated gpppg
    Unmethylated Gpppg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g+g+cap+analog/G(5)ppp(5)G+RNA+Cap+Struc+Analog/pm42103131-88-26-28
    Average 95 stars, based on 188 article reviews
    unmethylated gpppg - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    In Vitro:

    Article Title: The debranching enzyme Dbr1 regulates lariat turnover and intron splicing
    Article Snippet: .. 574 In vitro Dbr1 inhibition assay: 20 nM Dbr1 was combined with 0.2 uM AK88 and the concentrations of 575 G-G cap analog (NEB S1407), G-A cap analog (NEB S1406), and cGAMP (InvivoGen 576 nacga23).Product development was measured as described in the “Debranching assay and Western 577 blot” methods. .. Resulting curves were fit with Graphpad Prism to obtain IC50 values using the model 578 Y=Bottom + (Top-Bottom)/(1+(X/IC50)).

    Inhibition:

    Article Title: The debranching enzyme Dbr1 regulates lariat turnover and intron splicing
    Article Snippet: .. 574 In vitro Dbr1 inhibition assay: 20 nM Dbr1 was combined with 0.2 uM AK88 and the concentrations of 575 G-G cap analog (NEB S1407), G-A cap analog (NEB S1406), and cGAMP (InvivoGen 576 nacga23).Product development was measured as described in the “Debranching assay and Western 577 blot” methods. .. Resulting curves were fit with Graphpad Prism to obtain IC50 values using the model 578 Y=Bottom + (Top-Bottom)/(1+(X/IC50)).

    Western Blot:

    Article Title: The debranching enzyme Dbr1 regulates lariat turnover and intron splicing
    Article Snippet: .. 574 In vitro Dbr1 inhibition assay: 20 nM Dbr1 was combined with 0.2 uM AK88 and the concentrations of 575 G-G cap analog (NEB S1407), G-A cap analog (NEB S1406), and cGAMP (InvivoGen 576 nacga23).Product development was measured as described in the “Debranching assay and Western 577 blot” methods. .. Resulting curves were fit with Graphpad Prism to obtain IC50 values using the model 578 Y=Bottom + (Top-Bottom)/(1+(X/IC50)).



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    Image Search Results


    (A) A-cap / stemloop IRES reporter RNA carrying the negative control “scramble 1” sequence was mixed into a polysome lysate made from HEK293T cells for five minutes on ice and loaded on a sucrose gradient. (B) The sucrose gradient was fractionated and cDNA was prepared from groups of fractions as shown (numbered 1 -6). qRT-PCR was performed to quantify the exogenous “scramble 1” RNA across the fractions, as compared to endogenous Gapdh mRNA. The “scramble 1” transcript migrated into polysomal fractions 4-6, even though it is not translated .

    Journal: bioRxiv

    Article Title: Evaluating the reliability of tools for mRNA annotation and IRES studies

    doi: 10.64898/2026.03.29.707813

    Figure Lengend Snippet: (A) A-cap / stemloop IRES reporter RNA carrying the negative control “scramble 1” sequence was mixed into a polysome lysate made from HEK293T cells for five minutes on ice and loaded on a sucrose gradient. (B) The sucrose gradient was fractionated and cDNA was prepared from groups of fractions as shown (numbered 1 -6). qRT-PCR was performed to quantify the exogenous “scramble 1” RNA across the fractions, as compared to endogenous Gapdh mRNA. The “scramble 1” transcript migrated into polysomal fractions 4-6, even though it is not translated .

    Article Snippet: The ARCA m 7 G-cap analog (New England Biolabs; S1411) was used at a 4:1 ratio to GFP for transcription of T7-nluc constructs, while an A-cap analog (New England Biolabs S1406), was used to generate T7HP-nluc IRES reporter RNAs.

    Techniques: Negative Control, Sequencing, Quantitative RT-PCR